Technology in medicine has taken a new dimension. With regards to testing for presence of diseases and other anomalies, Elisa technology has taken over. The cardiac Elisa kits have been particularly so good. They are devices capable of working with the hearts of almost all animals in the world to establish any defects on it.
The process of diagnosing illnesses depends on an enzyme reaction that gives its results through exhibiting certain color changes. The enzyme and some antibodies combine with antigens, and subsequently change their color to indicate occurrence of a reaction. With this test, researchers are able to detect both antigens and antibodies.
This exercise is capable of detecting antibodies and antigens in patients. This is very useful; it helps detect illnesses before they develop into chronic problems. Doctors are able to work on it during its early stages of development. The patient will, therefore, be able to eliminate the problems at an affordable value.
Proper working of this equipment means it is sensitive to reactions, gives accurate results, and is capable of making many detailed readings at a time. When a tool is sensitive, it can exhibit any slight change resulting from the reaction between samples and reagents. Its accuracy ensures that results obtained are free of errors, and hence, believable. They are also manufactured to work on specific problems.
Stability is also important in the working of this equipment. To ensure stability, the loss rate of the activity has to be kept as low as possible. Good storage is also very important in ensuring stability. For the purpose of minimizing the effects of the environment on this experiment, standard lab conditions are very important; room temperature, standard pressure and humidity. The temperatures within the incubator should be closely regulated. It is also important to have one person working on the experiment from beginning to end.
For proper working of this activity, the researcher must ensure that all standards, reagents and samples are prepared in advance. The next thing is addition of some samples to all the wells and incubating them for close to 2 hours. He should then aspire and add some reagents. Next, he should put it back in the incubator for an hour, and later aspire and wash the mixture three times. The substrate solution should then be added, and the mixture incubated for a period of between 20 and 25 minutes. After, this step, a stopping agent must be added to stop the reaction for the researcher to make readings.
The enzyme sandwich principle is applied in this experiment. Plates on the kits are coated in advance with specific antibodies for the problem under investigation. Standards or samples are then appropriately added to the plates. They normally contain antibodies which are specific to certain defects. Lastly, Avidin conjugate is put on each plate and then incubated.
After putting substrate solutions together with other reagents, only the micro-wells will have Tropin I type three. A color change will then be exhibited, and a stopper solution is added. The change in color is then measured using wavelengths.
The process of diagnosing illnesses depends on an enzyme reaction that gives its results through exhibiting certain color changes. The enzyme and some antibodies combine with antigens, and subsequently change their color to indicate occurrence of a reaction. With this test, researchers are able to detect both antigens and antibodies.
This exercise is capable of detecting antibodies and antigens in patients. This is very useful; it helps detect illnesses before they develop into chronic problems. Doctors are able to work on it during its early stages of development. The patient will, therefore, be able to eliminate the problems at an affordable value.
Proper working of this equipment means it is sensitive to reactions, gives accurate results, and is capable of making many detailed readings at a time. When a tool is sensitive, it can exhibit any slight change resulting from the reaction between samples and reagents. Its accuracy ensures that results obtained are free of errors, and hence, believable. They are also manufactured to work on specific problems.
Stability is also important in the working of this equipment. To ensure stability, the loss rate of the activity has to be kept as low as possible. Good storage is also very important in ensuring stability. For the purpose of minimizing the effects of the environment on this experiment, standard lab conditions are very important; room temperature, standard pressure and humidity. The temperatures within the incubator should be closely regulated. It is also important to have one person working on the experiment from beginning to end.
For proper working of this activity, the researcher must ensure that all standards, reagents and samples are prepared in advance. The next thing is addition of some samples to all the wells and incubating them for close to 2 hours. He should then aspire and add some reagents. Next, he should put it back in the incubator for an hour, and later aspire and wash the mixture three times. The substrate solution should then be added, and the mixture incubated for a period of between 20 and 25 minutes. After, this step, a stopping agent must be added to stop the reaction for the researcher to make readings.
The enzyme sandwich principle is applied in this experiment. Plates on the kits are coated in advance with specific antibodies for the problem under investigation. Standards or samples are then appropriately added to the plates. They normally contain antibodies which are specific to certain defects. Lastly, Avidin conjugate is put on each plate and then incubated.
After putting substrate solutions together with other reagents, only the micro-wells will have Tropin I type three. A color change will then be exhibited, and a stopper solution is added. The change in color is then measured using wavelengths.
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