Tuesday, March 26, 2013

Basics Of A Real Time PCR Kit

By Tamra Ferrell


A real time pcr kit is a product that is useful in detecting and quantifying a dna molecule. It is based on the polymerase chain reaction where the dna will be sequenced as it is reacting in real time. This type of method may use fluorescent dyes or probes to quantify the sample. This is a highly useful research and diagnostic tool.

It has been useful in the medical community because it can help in diagnosis. When patients come in for treatment, it may not be immediately obvious what has caused their illness. By having a test done using this product, the correct diagnosis can be provided. This is also a tool used to observe new types of illnesses.

Another application is in the food industry. Safety has always been a concern because food items are being produced in mass quantities. If the processes are not adequate to prepare the food, a lot of people can get sick. Researchers can use it to detect pathogens and observe the reaction in the food. It can also be used for assessing liquids.

It is a useful tool for research, given the range of organisms that are being studied. It takes research to whole new level because a virus, for example, can be assessed on the dna level. Scientists can observe the changes in the organism when the environment changes. They can even determine how the gene is being expressed.

There is another method that relies on the polymerase reaction but there is one major difference. Real time means that the reaction is observed when it is happening. The traditional method on the other hand observes the reaction once it is finished. This limits the results and more processing may be necessary.

You are not sacrificing the sensitivity of your equipment. The results will still be reliable and at par with the results obtained from traditional equipment. The test will be performed in a closed environment so that there is less risk of contaminating the samples inside. Using such equipment is also not hard.

Different systems are available in the market and they work by different mechanisms. The choice of system will determine the accuracy of the results so the buyer should take the time to examine the features and what the product offers. Consider the cost of the reagents and how easily it can be used. Find out how much time is needed to get results so you can finish your analysis quickly. Moreover, check the software that will be used to illustrate the results.

It is important to buy the product from a reputable company. Avoid substandard products and services by checking the credentials and reputation of the companies you are considering. In addition, check if they can continue providing their service after your purchase if for example you start experiencing issues with the product.

A real time pcr kit is a tool that can be used for many applications. It is generally a product that is used to detect and quantify dna material by using the polymerase chain reaction. This ability makes it easier for researchers to observe a variety of organisms. The products can be easily accessed from retailers. Understand the different systems being used so you know which product is suitable for your purpose.




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